| Although synthetic insecticides are proved to be | | | | filtrate is evaporated to dryness. Concentrated |
| effective in pest control its frequent use causes | | | | extract was tested for its inhibitory effect on egg |
| major problems such as health hazards either by | | | | hatching of Aedes aegypti and compared with water |
| inhalation or ingestion of pesticide or its residue along | | | | as control and temephos(50%EC) as standard |
| with the food crop. Due to the public concern over | | | | synthetic insecticide. In another experiment, eggs |
| the toxicity & environmental impact of | | | | were allowed to exposure of calcium hypochlorite |
| conventional synthetic insecticides a novel kind of | | | | and acetic acid for 30min. to render the egg chorion |
| mosquito control is necessary. Genitically modified | | | | transparent, without affecting embryo morphological |
| mosquitoes & exploration and utilization of | | | | integrity. This enabled observation of embryo |
| naturally occurring products for combating harmful | | | | development under low power of binocular light |
| mosquitoes are the two possible ways to attain this | | | | microscope . Embryos at typical major developmental |
| goal. It turns out that analysis of the embryonic | | | | stages were clicked through microscope camera. |
| development of wild type mosquitoes is important in | | | | At the end of embryogenisis the embryo is clearly |
| order to accomplish exogenous DNA injections, | | | | divided in head, thorax and a segmented abdomen. |
| Phenotypic analysis of mutants and even to define | | | | This work aims at filling a gap on mosquito |
| the expression pattern of new genes. However | | | | embryogenisis knowledge, necessary as a toll in |
| when culicidae species are concerned, morphological | | | | further studies directed towards the control of |
| information on their developmental biology is limited. | | | | mosquitoes. |
| Therefore we decided to analyze Aedes aegypti | | | | The extract from the leaves of Annona Squamosa is |
| embryonic development. | | | | a good inhibitor of mosquito breeding as it is |
| Further in order to take important step in exploration | | | | environmentally safe and non toxic to humans as |
| and utilization of naturally occurring products for | | | | compared to temephos (50%EC).There is need for |
| combating harmful mosquitoes, we evaluated leaves | | | | studies that will provide the basis for developing |
| extract of Annona squamosa for its inhibitory effect | | | | more effective prevention stratigies, including |
| on the embryonic development of aedes aegypti | | | | bioenvironmental factors responsible for mosquito |
| mosquito, a most common vector of dengue virus in | | | | breeding and new mosquito control measures. |
| tropical countries. Dried, powdered leaves of Annona | | | | We hope this study would enable such an approach. |
| squamosa were macerated in methanol for 24hrs., | | | | |