| 1]User should take extra care while handling the toxic | | | | pipetting at temperature different from ambient. |
| or infectious liquids to prevent coming in direct | | | | • Wipe the tip only if there is liquid on the outside |
| contact with these liquids. Usage of hand gloves, | | | | of the tip, being careful to avoid touching the |
| apron and proper face mask is recommended as a | | | | tip’s orifice. |
| precaution against the accidental spillage over the | | | | • Don’t keep pipette in your hand while not |
| body parts. | | | | working to avoid transferring of body heat to |
| 2]The operating button should be pushed/released | | | | pipette. |
| back slowly and steadily at all the times. Taking extra | | | | • Select the correct pipetting technique(e.g, |
| care while working with liquids of high viscosity. Never | | | | Reverse, forward etc.) depending on the nature of |
| let the operating button snap back to the Ready | | | | the liquid. |
| position while aspirating the liquid. | | | | 4] |
| 3] | | | | • Using excessive force to turn the push button |
| • Aspirate liquid into the pipette only when a | | | | outside the range specified for it may jam the |
| proper tip is attached to its tip-cone. | | | | mechanism and damage the pipettor. |
| • Always clean the tip cone with a dry tissue | | | | • Re-calibration is recommended every 6 months |
| paper before fixing the tip. | | | | for a pipette used on daily basis. Re-calibration is also |
| • Ensure that the tip is tightly fitted to the tip | | | | recommended in the following cases |
| cone. | | | | 1. When working with detergents or foaming liquids. |
| • Reject the tip if any liquid remains sticking on | | | | 2. When working with high viscosity liquids. |
| the inner wall of the tip even after the plunger is | | | | 3. In the event of dismantling of the pipette for |
| pushed to the second stage of pushbutton. | | | | servicing, greasing, O-ring replacement etc. |
| • While pipetting , the pipettor should be vertically | | | | • Use disposable tips only once to avoid |
| straight and tip should be dipped only a few | | | | cross-contamination. |
| millimeters into the liquid. | | | | Avoid using inferior quality tips to ensure accurate |
| • Pre-rinsing of tip 5 times with the liquid to be | | | | dispensing of the liquids. |
| dispensed is recommended while doing the pipette | | | | • When not in use the pipettor should be rested |
| calibration. This is important especially when dispensing | | | | upright on the pipette stand in the vertical position |
| the liquids which have a viscosity and density | | | | with the tip cone pointing downwards to prevent |
| different from water. | | | | liquids entering the inside of the pipettor. |
| • Always control the push button movements | | | | • The micropipette user has to be fully aware of |
| with the thumb for consistency. | | | | the hazards within the samples. Spillage, inhalation or |
| • Allow liquids, Tips and pipettor to equilibrate with | | | | contact with human body are dangerous. The liquid |
| the ambient temperature. | | | | handling element of the procedure should present as |
| • Pre-rinse the tip several times before use when | | | | few hazards as possible to the user. |