| Growth of Micro organisms: | | | | Pure culture of bacteria is needed to study their |
| Outcomes: | | | | characteristics.Single colonies are produced to know |
| In this article I will be discussing about bacterial | | | | whether is pure or not. |
| growth. | | | | Methods of producing single colonies are |
| Microbial Growth: It can be defined as an increase in | | | | 1. Streaking or spreading method |
| cellular constituents which may result in increase of | | | | 2. Pour plate method. |
| size of the micro organism and its population number. | | | | Stages in a microbial growth: |
| Bacterial multiplication: bacteria multiply by binary | | | | There are four stages in microbial growth they are |
| fission, conjugation etc.., | | | | 1. Lag phase |
| Binary fission is a method in which bacteria multiply in | | | | 2. Log phase |
| doubles like from 1 to 2 and from 2 to 4 and 4-8 so | | | | 3. Stationary phase |
| on. | | | | 4. Death phase. |
| What do these bacteria grow on: Generally bacteria | | | | THE CONTINUOUS CULTURE OF MICRO |
| grow on a culture media. The culture media consists | | | | ORGANISMS: |
| of all the nutrient media required for growth. | | | | These are used to maintain the cells in exponential |
| Culture media: culture media can be of either liquid or | | | | growth phase at a constant biomass concentration |
| solid forms. | | | | for a extended period of time. |
| Liquid media: This can be solidified by addition of agar. | | | | There are two kinds of continuous, culture systems, |
| Agar is not a nutrient media; it is only a solidifying | | | | chemostat and turbidostat. |
| agent. | | | | Bacterial growth can be defined as an increase in the |
| Pure culture: | | | | micro-organism's cell size or the population number. |