What is Ultraviolet Microscopy?

As already seen in “Principles of Microscopes andconclude that ultraviolet microscopy enables a useful
Microscopy (Part I)”, light microscopy can bemagnification of about twice the magnification of
divided into six sub-categories: bright field microscopy,bright field microscopy.
dark field microscopy, ultraviolet microscopy,In addition to promoting a larger magnification,
fluorescence microscopy, phase contrast microscopywithout prejudice of clearness, the ultraviolet
and differential interference contrast (D.I.C).microscopy also allows the observation of substances
The so-called ultraviolet microscopy uses ultravioletabsorbed for microorganisms, which become visible
light instead of white, common, light, or visible light,as ultraviolet light reaches upon them and make them
as the light source. The ultraviolet light has afluorescent. This is the main application of ultraviolet
wavelength of 180 to 400 nm, much smaller than themicroscopy, I mean, histochemical.
visible light, whose wavelength is from 400 to 700The ultraviolet microscope differs from the
nm. And the advantage is?conventional microscope, for since ultraviolet
Let’s recap how the resolution power isradiations are not visible, images need to be
calculated:impressed on a photographic film, by use of an image
Resolution power = light wavelength / NA objectiveconverter tube, or by the projection on a screen,
+ NA condenserright after being captured by a phototube. In addition,
According to the above equation, the smaller theultraviolet microscopy needs special lens for the
wavelength, smaller the resolution power, whattransmission of ultraviolet light and optic resources to
means you will be able to observe even smallerreflect the region of interest, 230 to 350 nm.
objects through the microscope. That said, we can